• Another group identified the same phosphorylation sites in t

  2018-10-20

  

  Another group identified the same phosphorylation sites in the Nanog protein (22). However, they did not show a direct phosphorylation of Nanog but showed that it was phosphorylated at multiple Ser/Thr-Pro motifs by an unidentified kinase. We showed the direct phosphorylation of Nanog by ERK1 (Fig. 

• Loss of the ApcWT allele in intestinal cells in organoid

  2018-10-20

  

  Loss of the ApcWT allele in intestinal met inhibitor in organoid culture induces Wnt target genes and high self-renewal potential in these cells upon which they give rise to neoplastic growth. This manifests in cyst-like organoids with aberrant tissue architecture, cell differentiation and prolifera

• The following are the supplementary data related to this

  2018-10-20

  

  The following are the supplementary data related to this article. Acknowledgments This work was supported by the Andrew L. Warshaw, MD Institute for Pancreatic Cancer Research, Massachusetts General Hospital, and by NIH-P01CA117969 to SPT. We would like to Thank Dr. Timothy Wang, Dorothy L. and

• latrunculin manufacturer br Materials and methods br Verific

  2018-10-20

  

  Materials and methods Verification of karyotype Acknowledgments This project was funded by the Department of Biotechnology, Government of India (grant no. BT/IN/Denmark/31/MSI/2013) and the Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore. Resource Table: Resource d

• MK-571 sodium salt hydrate br Resource table Resource detail

  2018-10-20

  

  Resource table: Resource details Expanded T MK-571 sodium salt hydrate isolated from a 38year-old Japanese healthy male were reprogrammed employing Sendai virus vectors (SeVdp) expressing four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4. SeVdp is integration-free vector, and the absence of

• br Materials and methods br Results br

  2018-10-20

  

  Materials and methods Results Discussion We have identified and begun to characterize a novel miRNA, miR-410, which on inhibition of BMP signaling by Noggin, is down-regulated in the adult SVZ. Over-expression of miR-410 in SVZ neurospheres inhibited neuronal differentiation and increased t

• cam kinase ii To test whether the direction of the

  2018-10-20

  

  To test whether the direction of the genomic strand targeted by the gRNA molecule was a factor in its capacity to recruit dCas9 and activate expression of the endogenous locus, we designed a gRNA molecule (gRNA4) targeting the minus strand of the same region for which gRNA3 was designed (PAM separat

• br Experimental Procedures br Author

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction Fragile X syndrome (FXS) affects approximately 1 in every 4,000 boys and 1 in 8,000 girls worldwide (Callan and Zarnescu, 2011; Penagarikano et al., 2007). It is now believed that FXS is the leading cause of inheri

• The trend for increased differentiation in the field is

  2018-10-20

  

  The trend for increased differentiation in the field is paralleled by a large and increasing proportion of papers in which both cell types are being used. To analyze the validity of the “gold standard” argument that is frequently used to justify the continued use of hESCs in research, we analyzed th

• Transplantation of iPSC NSCs within the neonatal

  2018-10-20

  

  Transplantation of iPSC-NSCs within the neonatal sigma receptor yielded stable engraftment across the neuroaxis for at least 4 months, but only within and adjacent to white matter tracts. Although the precise mechanisms are unclear, NSCs from various sources display a tropism for white matter in bo

• Renal capsule implantation of Lgr cells further supported

  2018-10-20

  

  Renal Talabostat mesylate implantation of Lgr5+ cells further supported the high repopulation capacity of single Lgr5+ cells within the regressed prostate. A limiting-dilution analysis indicated that every prostatic Lgr5+ cell could be a stem cell in regressed prostates, meaning that both luminal a

• br Heteroplasmy mitochondrial segregation and disease In

  2018-10-20

  

  Heteroplasmy, mitochondrial segregation and disease In an individual with a mitochondrial disease, different cells or populations may have differing amounts of the mutant mitochondria, ranging from complete homoplasmy – individuals with all mutant mitochondrial DNA as occurs in Leigh\'s Syndrome

• br Materials and methods br Resource table

  2018-10-20

  

  Materials and methods Resource table Material and methods Introduction While increasingly effective for treating childhood cancers, chemotherapeutic agents and radiotherapy are known to have deleterious effects on the gonads of prepubertal boys (Schrader et al., 2001; Wallace, 2011). As

• br Acknowledgements We extend our sincere thanks to Cindy Ch

  2018-10-20

  

  Acknowledgements We extend our sincere thanks to Cindy Cheung for her help with the immunostaining, Yuhuan Wang for her help with daily cell culture, and Atena Zahedi for growing the GFP-RAC activated plasmid. The pcDNA3-EGFP-Rac1-Q61L plasmid (Plasmid # 12981) was created by Gary Bokoch and purc

• br Introduction Alkaline phosphatase ALP is a ubiquitous mem

  2018-10-20

  

  Introduction Alkaline phosphatase (ALP) is a ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values. Examination of expression of ALP at intracellular and extracellular level is a widely-used procedure in both clinical practice and basic re

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